Cup Plate Method | Cultivation Of Microorganism Essay

Cup Plate Method | Cultivation Of Microorganism Essay

An Adduct formed by stirring (0.01 mole) of aromatic aldehyde with the 40% of NaHSO3. O-phenylenediamine (0.01 mole) was dissolved in 50 ml of warm Ethanol 80. The NaHSO3 adduct of the aldehyde is added slowly with constant stirring in the warm solution of O-phenylenediamine stirred for 20-30 min still solid product obtained, then added 100 ml of Distilled water and filtered . Now the product was recrystallised by using Ethanol.


Step 2: Nicotinoyl Cloride

0.1 mole of Nicotinic Acid was refluxed for 6 hrs with the 20 ml of Thionyl Chloride. After this the excess of Thionyl Chloride was distilled off and separated from the product and dried it.

Step 3:

0.01 mole of 2-phenylbenzimidazole solution in 100 ml Pyridine stirred for 8 hrs constantly with the 0.01 mole of Nicotinoyl Chloride ,then the water added 50 ml to get a solid product. The product was filtered, dried and recrystallised using Ethanol. Cup Plate Method | Cultivation Of Microorganism Essay.



Molecular modification of a promising lead compound is still a major line of approach for the discovery of new drug. Molecular modification involves substituting, elimination, or adding new moieties to a parent lead compound, there by making gradual changes in the physico-chemical properties of the parent compound and thus biological activity of the compound.

It is clear from the literature review that a number of Benzimidazole derivatives are known for the, antibacterial, antifungal and ant-inflammatory activities properties.

The present studies were performed with the following objectives:

  • Synthesis of new series of 1,2-substituted benzimidazole derivatives.
  • Characterization of newly synthesized compounds by spectra methods viz.infrared spectra (IR spectra), Nuclear magnetic resonance spectra (¹H NMR spectra) and (Mass spectra). Cup Plate Method | Cultivation Of Microorganism Essay.
  • Screening of the antibacterial and Antifungal of the newly synthesized compounds using various strains of bacteria and fungi by determining their MIC.
  • Screening of anti-inflammatory action of Benzimidazole derivatives. Cup Plate Method | Cultivation Of Microorganism Essay.