Bacteria Growth and Plasmid Retention in E Coli Assignment Paper

Bacteria Growth and Plasmid Retention in E Coli Assignment Paper

Bacteria Growth and Plasmid Retention in E Coli Introduction

The increasing interest by the industry in recombinant protein production (RPP), due to the number of applications it can provide, has caused an intensive study in this area in order to improve its protocols. Thus, an improvement would make possible an increment in the target protein yield and the quality production as well as to establish more efficient host and plasmid for each target protein .Bacteria Growth and Plasmid Retention in E Coli Assignment Paper

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The most common hosts utilized in RPP are bacteria because of the capacity that they have to express almost any gen and the relative facility to modify and use their plasmids in order to produce the target protein .

However, it is widely known the number of problems that these hosts have when they produce a high amount of recombinant protein. Firstly, a frequent problem is the appearance of inclusion bodies which hinder a correct recovery of the target protein produced . Secondly, the host lysis event is the other common problem in RPP in bacteria. This undesirable happening in the production of recombinant proteins can be produced for several reasons. One of them is the high level synthesis of the mRNA and the target protein . Bacteria Growth and Plasmid Retention in E Coli Assignment Paper. Other reasons described are the accumulation of fragments of the recombinant protein because of the proteolysis . Finally, the main cause of the problems related to RPP is the accumulation of incorrectly folded intermediates of the recombinant protein. In E. coli this fact implies general stress responses .

In order to find a proper protocol for the protein S (PS) production in E. coli and know more about the RPP process, we conducted an experiment testing post-induction bacteria growth, production of target protein and plasmid retention. In this experiment the E. coli strain BL21*DE3 transformed with the plasmid pCV05 was used to express the PS fused with a C-terminal His tag (6xHis).

Bacteria Growth and Plasmid Retention in E Coli Assignment Paper