Titration of Amino Acids and Peptides Essay Assignment
Titration of Amino Acids and Peptides Essay Assignment
Titration of Amino Acids and Peptides: Titration is the process used to analyze the acid-base behaviour of amino acids and peptides. A 0.25 g sample of unknown amino acid and aspartame were transferred quantitatively to separate 250-ml Erlenmeyer flask. 20.0 ml of distilled water was added to each flask using a volumetric pipette then the flask was swirled to dissolve the samples. Small increments of 0.200 M HCl were added to both samples to produce an acidic solution and then each sample was titrated by adding 0.200 ml of 0.200 M NaOH. The pH of the acidic solutions was measured from 1.5 until it reaches pH 11 and it was recorded in every addition of the base. Titration of Amino Acids and Peptides Essay Assignment. On the Aspartame, the total volume of the base added was 8.0 ml and 29.6 ml on the unknown amino acid. Titration curves were constructed based on the pH values and volume added. The identity of the unknown amino acid was revealed to be Aspartic acid. A titrimetric profile of the amino acid and aspartame were drawn.
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INTRODUCTION
Proteins are the most important class of biochemical molecules, next are carbohydrates and lipids. Proteins are the basis for the major structural components of animal and human tissue. Proteins are also the indispensable agents of biological function and amino acids are the building block of proteins. Titration of Amino Acids and Peptides Essay Assignment. Amino acid is an organic compound containing an amino group (-NH2) and a carboxyl group (-COOH). Since amino acids contain both an acidic and a basic group, they undergo an intra molecular acid-base reaction and exist primarily in the form of a dipolar ion, or zwitterions. Amino acids are also amphoteric, meaning, they can react either as acid or base depending on the circumstances. There are basically 20 primary amino acids that serve as the backbone of most proteins…..